ช่วยตรวจไวยากรณ์ให้ด้วยค่ะ

Anaplasma platys and Ehrlichia canis are important emerging tick-borne diseases, especially in companion animal.  Therefore, this research is purpose to study about A. platys and E. canis prevalence in stray cats.  Blood samples were collected from 216 stray cats living in 24 temples in Sampran district, Nakhon Pathom province.  Then, blood samples were divided into two parts; processed for buffy coat smear and microscopic finding for the detection of A. platys and E. canis inclusion bodies, and processed for DNA-extraction.  DNA were subjected to polymerase chain reaction (PCR) for the detection of both A. platys and E. canis, A. platys specific PCR, E. canis specific PCR, and positive PCR products were submitted for DNA sequencing consequently.  The microscopic finding results showed nine samples positive for Brugia spp. microfilariae.  PCR results were demonstrated only positive for A. platys and E. canis PCR, whereas DNA sequencing results were showed Paracoccus spp. and Mesorhizobium spp.  We proposed that the samples might be contaminated from non-sterilized sample collection procedure or device and a non-specific binding between primer and Paracoccus spp. and Mesorhizobium spp. in PCR procedure was occurred.